Saccharomyces cerevisiae is widely used in food and beverage production but faces stresses such as acidity during processing. In our previous studies, the S. cerevisiae mutant strain TAMC, which can tolerate an acidic environment of pH 2.3, was isolated via adaptive laboratory evolution; however, the genetic basis underlying the acid tolerance of this strain remained unclear. Herein, we aimed to elucidate the genetic basis of TAMC's acid tolerance via genome sequencing and functional analysis. The results revealed that a mutation (mROX1) in transcriptional regulator ROX1 was crucial for acid tolerance. Comparative transcriptome mining and reverse engineering revealed that the mROX1 mutation mediated cellular oxidation-reduction stress homeostasis maintenance by upregulating the expression of superoxide dismutase homologues and catalase, facilitating tolerance to acidity and detoxifying reactive oxygen species. This work uncovers a novel yeast intracellular homeostasis mechanism, providing a potential strategy for engineering novel acid-tolerant microbial cell chassis.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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