The development of herbicide-resistant crops is a key strategy for achieving efficient and sustainable agriculture. Herbicide tolerance can be conferred by transporters that mediate the efflux of herbicides, making them of particular interest for resistance development. However, identifying a transporter for a specific compound can be a challenging task. Here, we demonstrate the use of a synthetic, yeast two-hybrid-based molecular biosensor to identify transporter activity. A previously engineered biosensor responsive to the herbicide alachlor was utilized to monitor cytosolic alachlor levels in yeast, allowing for the evaluation of candidate transporters. A biosensor-reported shift in alachlor accumulation indicated the Saccharomyces cerevisiae transporter PDR5 as a potential mediator of alachlor efflux. PDR5's effect on alachlor accumulation was suppressed by a known competing substrate of the transporter, validating its alachlor efflux activity. The ability of the biosensor yeast platform to report transporter activity and its inhibition via a fluorescence output underscores its potential as a tool for transporter-focused research.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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