Autophagy is an important mechanism for maintaining cell homeostasis, and its decline in function is widely regarded as a key factor in aging. Catalpol (CAT), an active compound derived from the traditional Chinese herbRehmannia glutinosa, has shown potential to treat various age-related diseases through the regulation of autophagy. However, how CAT exerts anti-aging effects by regulating autophagy remains unclear. Therefore, this study sought to explore the anti-aging potential and molecular mechanisms, particularly the role of CAT in autophagy and mitochondrial function in yeast cells. The PI-CLS measurement method was employed to demonstrate enhanced yeast cells viability upon CAT treatment. RNA-Seq combined with bioinformatics analyses identified HSP82 as a key target of CAT. HSP82 knockout and HSP82 overexpression strains were employed to demonstrate that CAT enhances mitochondrial functions and extends cells lifespan by inducing HSP82-dependent mitophagy. We concluded that CAT plays an anti-aging role by regulating HSP82-dependent mitophagy and mitochondrial functions in yeast cells.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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