The nucleolus is a non-membrane-bound compartment that forms around tandem arrays of ribosomal RNA genes (rDNA) and provides the cell with ribosomes. Multiple nucleoli within the same nucleus coalesce, and fusion is thought to result mainly from intrinsic properties of nucleoli. However, rDNA arrays are mostly in chromosomal context, and chromosomes are not randomly organized. How the spatial arrangement of chromosomes affects nucleolar fusion is largely unknown. Using fluorescence microscopy, we investigated nucleolar fusion in diploid budding yeast. Nucleoli forming around homologous rDNA arrays efficiently fused during interphase but often individualized during late anaphase. While nucleoli were far from the spindle pole body (SPB) in interphase, they came close during mitosis suggesting that SPB-dependent positioning may affect nucleolar fusion. Indeed, disruption of microtubule-dependent centromere anchorage to the SPB by nocodazole promoted individualization of nucleoli. In contrast, impairment of rDNA tethering to the nuclear envelope had little or no effect. Hence, chromosome positioning by non-rDNA sequences facilitates nucleolar fusion.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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