Higher alcohols are significant flavor compounds in wine, and the elevated sugar content in grape raw materials has a substantial impact on wine quality. This study investigates the effect of high sugar content on the production of higher alcohols in wine and elucidates the underlying mechanisms through transcriptome analysis. The results indicate that sugar concentrations of 240 g/L and 280 g/L lead to increases in higher alcohol content of 17% and 24%, respectively. Transcriptome sequencing was employed to analyze differentially expressed genes at various fermentation stages, which resulted in the identification of the GRE3 gene. It was determined that the expression level of GRE3 significantly influences higher alcohol content. Knocking out GRE3 using molecular methods led to a notable 17.76% decrease in higher alcohol yield at a sugar concentration of 240 g/L, representing a novel finding not previously documented in the literature. This research provides valuable insights into the influence of high-sugar grape materials on the production of higher alcohols by Saccharomyces cerevisiae and the associated mechanisms.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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