The freeze-thaw cycle presents a significant challenge to Saccharomyces cerevisiae in industrial applications, impacting its resilience and viability. Traditional genetic modifications in industrial yeast strains have achieved limited success in enhancing freeze-thaw tolerance. To address this, we explored the untapped genetic diversity of wild diploid strains and developed a hybrid fusion evolution (HFE) strategy. This approach involved isolating haploids, implementing targeted modifications to increase glycerol accumulation and aquaporin expression, and subjecting these modified strains to cycles of fusion and selection. The HFE method enabled the development of robust yeast strains with significantly improved freeze-thaw tolerance, from an average of no more than 12% for the initial parents to an average of 67% for the third-generation strains, alongside enhanced resilience to other stresses such as high ethanol and moderate heat. Our findings demonstrate the potential of combining microbial breeding and gene regulation to exploit natural genetic diversity, providing a promising solution to improve industrial yeast strains for sustainable food production under environmental stress conditions.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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