The Lithium-PEG method for transforming yeast cells is a standard procedure used in most yeast laboratories. After several optimizations, this method can yield up to 10⁶ transformants per µg of plasmid. Some applications, such as library screening or complex transformations, necessitate maximizing transformation yield. Here, we demonstrate that the addition of a sorbitol solution serves as an osmo-protectant during and after heat shock, resulting in up to a tenfold increase in transformation efficiency. This optimization requires only one additional pipetting step compared to the original protocol, making it practical for routine use.

• PMID: 40088047
• DOI full text
• PubMed
• PubTator

Reference Type

Journal Article | Review

Authors

Kadouch M, Gaspin P, Marchal C, Castano S, Cullin C

Primary Lit For

Additional Lit For

Review For