Reference: Pandita M, et al. (2024) Genetic Interaction Between F-Box Encoding UCC1 and RRM3 Regulates Growth Rate, Cell Size, and Stress Tolerance in Saccharomyces cerevisiae. J Biochem Mol Toxicol 38(12):e70059

Reference Help

Abstract


Ucc1, an F-box motif-containing protein of Saccharomyces cerevisiae encoded by UCC1 regulates energy metabolism through proteasomal degradation of citrate synthase Cit2 and inactivation of the glyoxylate cycle when glucose is present as the main carbon source in the growth medium. Rrm3, a Pif1 family DNA helicase, encoded by RRM3 regulates the movement of the replication forks during the DNA replication process. Here in this study, we present evidence of binary genetic interaction between both the genes, UCC1 and RRM3, that determine the growth rate, cell morphology, cell size, apoptosis, and stress response. The absence of both genes UCC1 and RRM3 leads to altered cell morphology, increased growth rate, utilization of alternate carbon sources, resistance to hydrogen peroxide, and susceptibility to acetic acid-induced apoptosis. Further, the genetic interaction network analysis shows both the genes UCC1 and RRM3 interaction through the SGS1 and cross-link among metabolic, glyoxylate, DNA replication, and retrograde signaling pathways.

Reference Type
Journal Article
Authors
Pandita M, Shoket H, Kumar R, Bairwa NK
Primary Lit For
RRM3 | UCC1
Additional Lit For
SGS1

Phenotype Annotations 1 entry for 1 gene


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
RRM3growth in exponential phase: decreased rate
classical genetics null
Allele: rrm3-Δ
S288CDetails: slow growth relative to wt; growth rate is elevated in the rrm3-Δ ucc1-Δ double mutant
Showing 1 to 1 of 1 entries