Palmitoleic acid (POA) has been widely applied to nutrition and pharmaceutical industry. However, high cost of scale-up fermentation restricts the extensive application of POA. Hence, we investigated the availability of corn stover hydrolysate (CSH) as carbon source in POA production by engineered S. cerevisiae. Although the yeast growth was inhibited to some extent by CSH, the POA production with CSH was slightly higher than that with pure glucose. The C/N ratio of 120 and addition of 1 g/L lysine raised the POA titer up to 2.19 g/L and 2.05 g/L, respectively. Two-stage cultivation could increase the POA titer by upregulating the gene expression of key enzymes in fatty acid synthesis pathway. A high POA content of 57.5% (v/v) and a highest POA titer of 6.56 g/L were achieved under the optimized conditions. These findings provide a feasible approach for sustainable production of POA or its derivatives from CSH.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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