Reference: Gardner JM, et al. (2021) A mutation in budding yeast BRR6 affecting nuclear envelope insertion of the spindle pole body. MicroPubl Biol 2021

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Abstract


BRR6 and BRL1 are two paralogs that encode transmembrane proteins of the nuclear envelope (NE) involved in membrane fluidity and nuclear pore complex biogenesis in organisms that undergo a closed mitosis. We show that mutation of a conserved cysteine in the intralumenal domain of Saccharomyces cerevisiae Brr6p results in a novel temperature sensitive allele, brr6-Y100H, that arrests growth due to defects in spindle formation. Analysis of brr6-Y100H cells by electron tomography and Brr6p localization by super-resolution imaging supports the idea that Brr6p is involved in insertion of the newly duplicated spindle pole body into the NE.

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Journal Article
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Gardner JM, O'Toole E, Jaspersen SL
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