ε-poly-L-lysine treated yeast (PTY) helps to enhance the freeze-thaw tolerance of frozen dough. This study explored the effect of PTY on the aggregation and the gas-retention of frozen dough after steaming for 2, 5, 10, and 20 min. Gas-cell images showed that PTY reduced the loss of gas-retention ability caused by 4 times of freeze-thaw. The results of sodium dodecyl sulfate extractability and subunit distribution of gluten showed that, after the same heat time, frozen dough with PTY has higher degree of covalent crosslinking with better aggregation ability of α-, γ-gliadin, and low molecular weight glutenin subunits than frozen dough with yeast. Chemical analysis and chain morphology results demonstrated that the levels of acidity, NH2, and free sulfhydryl in dough were decreased, and the protein molecules aggregated into longer chains when using PTY instead of yeast, indicating that PTY reduced acid-mediated hydrolysis and increased the disulfide bonds-mediated gluten polymerization.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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