Reference: Small EM, et al. (2020) SPOCK, an R based package for high-throughput analysis of growth rate, survival, and chronological lifespan in yeast. Transl Med Aging 4:141-148

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Abstract


Plate reader-based methods for high-throughput measurement of growth rate, cellular survival, and chronological lifespan are a compelling addition to the already powerful toolbox of budding yeast Saccharomyces cerevisiae genetics. These methods have overcome many of the limits of traditional yeast biology techniques, but also present a new bottleneck at the point of data-analysis. Herein, we describe SPOCK (Survival Percentage and Outgrowth Collection Kit), an R-based package for the analysis of data created by high-throughput plate reader based methods. This package allows for the determination of chronological lifespan, cellular growth rate, and survival in an efficient, robust, and reproducible fashion.

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Journal Article
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Small EM, Felker DP, Heath OC, Cantergiani RJ, Osley MA, McCormick MA
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