Reference: Vorländer MK, et al. (2020) Structural basis for RNA polymerase III transcription repression by Maf1. Nat Struct Mol Biol 27(3):229-232

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Abstract


Maf1 is a conserved inhibitor of RNA polymerase III (Pol III) that influences phenotypes ranging from metabolic efficiency to lifespan. Here, we present a 3.3-Å-resolution cryo-EM structure of yeast Maf1 bound to Pol III, establishing that Maf1 sequesters Pol III elements involved in transcription initiation and binds the mobile C34 winged helix 2 domain, sealing off the active site. The Maf1 binding site overlaps with that of TFIIIB in the preinitiation complex.

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Journal Article | Research Support, N.I.H., Extramural | Research Support, Non-U.S. Gov't
Authors
Vorländer MK, Baudin F, Moir RD, Wetzel R, Hagen WJH, Willis IM, Müller CW
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