Transient potential receptor (TRP) channels are conserved cation channels found in most eukaryotes, known to sense a variety of chemical, thermal or mechanical stimuli. The Saccharomyces cerevisiae TRPY1 is a TRP channel with vacuolar localization involved in the cellular response to hyperosmotic shock and oxidative stress. In this study, we found that S. cerevisiae diploid cells with heterozygous deletion in TRPY1 gene are haploinsufficient when grown in synthetic media deficient in essential metal ions and that this growth defect is alleviated by non-toxic Mn²⁺ surplus. Using cells expressing the Ca²⁺-sensitive photoprotein aequorin we found that Mn²⁺ augmented the Ca²⁺ flux into the cytosol under oxidative stress, but not under hyperosmotic shock, a trait that was absent in the diploid cells with homozygous deletion of TRPY1 gene. TRPY1 activation under oxidative stress was diminished in cells devoid of Smf1 (the Mn²⁺-high-affinity plasma membrane transporter) but it was clearly augmented in cells lacking Pmr1 (the endoplasmic reticulum (ER)/Golgi located ATPase responsible for Mn²⁺ detoxification via excretory pathway). Taken together, these observations lead to the conclusion that increased levels of intracytosolic Mn²⁺ activate TRPY1 in the response to oxidative stress.

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Journal Article | Research Support, Non-U.S. Gov't

Authors

Ruta LL, Nicolau I, Popa CV, Farcasanu IC

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