Ribosome biogenesis involves numerous preribosomal RNA (pre-rRNA) processing events to remove internal and external transcribed spacer sequences, ultimately yielding three mature rRNAs. Removal of the internal transcribed spacer 2 spacer RNA is the final step in large subunit pre-rRNA processing and begins with endonucleolytic cleavage at the C₂ site of 27SB pre-rRNA. C₂ cleavage requires the hierarchical recruitment of 11 ribosomal proteins and 14 ribosome assembly factors. However, the function of these proteins in C₂ cleavage remained unclear. In this study, we have performed a detailed analysis of the effects of depleting proteins required for C₂ cleavage and interpreted these results using cryo-electron microscopy structures of assembling 60S subunits. This work revealed that these proteins are required for remodeling of several neighborhoods, including two major functional centers of the 60S subunit, suggesting that these remodeling events form a checkpoint leading to C₂ cleavage. Interestingly, when C₂ cleavage is directly blocked by depleting or inactivating the C₂ endonuclease, assembly progresses through all other subsequent steps.

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Journal Article | Research Support, N.I.H., Extramural | Research Support, Non-U.S. Gov't

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Biedka S, Micic J, Wilson D, Brown H, Diorio-Toth L, Woolford JL

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