Using Saccharomyces cerevisiae as an experimental model, the potential toxicity of graphene oxide (GO) was evaluated following exposure to 0-600 mg L-1 for 24 h. The results showed that cell proliferation was observably inhibited and the IC50 value was 352.704 mg L-1. Mortality showed a concentration-dependent increase, and was 19.3% at 600 mg L-1. A small number of cells were deformed and shrunken after exposure. The percentage of late apoptosis/necrosis showed a significant increase (p < 0.01) at 600 mg L-1 (19.16%) compared with the control (1.14%). The mitochondrial transmembrane potential was significantly decreased (p < 0.01) at 50-600 mg L-1, indicating that the apoptosis was related to mitochondrial impairment. Moreover, ROS was observably increased (p < 0.01) at 200, 400 and 600 mg L-1. The expressions of apoptosis-related genes (SOD, Yca1, Nma111 and Nuc1) were significantly changed. The results presented so far indicate that GO has the potential to cause adverse effects on organisms when released into the environment.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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