Phospholipase D (PLD) is an important phospholipid hydrolase that plays critical roles in various biological processes in eukaryotic cells. However, little is known about its functions in plant pathogenic fungi. In this study, we identified three FgPLD genes in Fusarium graminearum that are homologous to the Saccharomyces cerevisiae Spo14 gene. We constructed deletion mutants of all three FgPLD genes using homologous recombination. Deletion of FgPLD1 (Δpld1), but not FgPLD2 or FgPLD3, affected hyphal growth, conidiation, and perithecium formation. The Δpld1 mutant showed reduced deoxynivalenol (DON) production and virulence in flowering wheat heads and corn silks. Furthermore, three FgPLD proteins have the same subcellular localization and localize to the cytoplasm in F. graminearum. Taken together, these results indicate that FgPLD1, but not FgPLD2 or FgPLD3, is important for hyphal growth, sexual or asexual reproduction, and plant infection.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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