Reference: Kwon Y, et al. (2016) A chemogenomic approach to understand the antifungal action of Lichen-derived vulpinic acid. J Appl Microbiol 121(6):1580-1591

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Abstract


Aim: To determine uncovered antifungal activity of lichen-derived compound, vulpinic acid, by using chemical-genetic analyses.

Methods and results: Haploinsufficiency and homozygous-profiling assays were performed, revealing that strains lacking GLC7, MET4, RFC2, YAE1 and PRP18 were sensitive to three concentrations (12·5, 25 and 50% of inhibitory concentration) of vulpinic acid and independently validated. To verify inhibition of those genes, cell cycle analysis using flow cytometry was performed and relative expressions were measured. Under vulpinic acid-treated condition, cell cycle was arrested in S and G2/M phases and sensitive strains' relative expressions were significantly lower than the wild type yeast.

Conclusions: Vulpinic acid mainly affects cell cycle, glycogen metabolism, transcription and translation to fungi.

Significance and impact of the study: Although lichen-derived compounds are commercially valuable, few studies have determined their modes of action. This study used a chemogenomic approach to gain insight into the mechanisms of one of well-known lichen-derived compound, vulpinic acid.

Reference Type
Journal Article
Authors
Kwon Y, Cha J, Chiang J, Tran G, Giaever G, Nislow C, Hur JS, Kwak YS
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