Mitotic cyclins drive initiation and progression through mitosis. However, their role during progression remains poorly understood due to their essential function in initiation of mitosis and redundant activities. The function of the principal mitotic cyclin, Clb2, in S. cerevisiae, was investigated during progression through anaphase in diploid cells after DNA damage and during normal growth using fixed and live cell fluorescence techniques. I find that during anaphase, absence of Clb2 affects chromosome movement and plays an important role in inhibiting kinetochore microtubules regrowth. In addition, absence of Clb2 leads to defects and the collapse of spindle pole body separation. Most unexpectedly, new bipolar spindle forms and spindle re-forms. The intensity of the defects appears to correlate with strength of checkpoint activation, and during adaptation to DNA damage, these defects lead to important chromosome missegregation, during normal growth, defects are resolved rapidly. During recovery, intermediate phenotypes are observed. Altogether, data reveal new and unexpected roles for mitotic cyclins during progression through mitosis; results indicate that mitotic cyclins play key role in growth suppression of kinetochore microtubules and suggest that new bipolar spindle formation might be actively inhibited by mitotic cyclins during anaphase.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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