Objective: To explore the individual roles of G protein beta and gamma subunit in the interactions with effectors.

Methods: We investigated the interactions of G beta 1 and G beta 1 gamma 2 with adenylyl cyclase II(AC II) using the yeast two-hybrid and three-hybrid systems.

Results: When assayed for the abilities to activate the reporter gene, the interactions among AD-beta 1, gamma 2 and BD-AC IIQ in the three-hybrid system were more potent than the interactions between AD-beta 1 and BD-AC II Q in the two-hybrid system. The expressions of BD-AC IIQ and AD-beta 1 in transformants coexpressed AD-beta 1 and BD-AC IIQ, and transformants coexpressed AD-beta 1, gamma 2 and BD-AC IIQ were respectively detected. The comparisons between the reporter activity and the expression levels of BD-AC IIQ and AD-beta 1 in the yeast cells show there was no correlations, i.e. The difference in the reporter activity was not a reflection of differential expression level of the hybrid proteins.

Conclusions: All these results suggest that G protein beta 1 subunit is sufficient to maintain the basic interaction between G beta 1 gamma 2 and AC IIQ, and gamma 2 subunit plays an important role in the high affinity interaction of G beta 1 gamma 2 with AC IIQ.

• PMID: 12905884
• PubMed
• PubTator

Reference Type

English Abstract | Journal Article | Research Support, Non-U.S. Gov't

Authors

Li JJ, Lu N, Huang BR

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