Crystallization has long been regarded as one of the major bottlenecks in high-throughput structural determination by X-ray crystallography. Structural genomics projects have addressed this issue by using robots to set up automated crystal screens using nanodrop technology. This has moved the bottleneck from obtaining the first crystal hit to obtaining diffraction-quality crystals, as crystal optimization is a notoriously slow process that is difficult to automatize. This article describes the high-throughput optimization strategies used in the Yeast Structural Genomics project, with selected successful examples.

• PMID: 15930617
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Reference Type

Journal Article | Research Support, Non-U.S. Gov't

Authors

Leulliot N, Trésaugues L, Bremang M, Sorel I, Ulryck N, Graille M, Aboulfath I, Poupon A, Liger D, Quevillon-Cheruel S, ... Show all

Primary Lit For

Additional Lit For

YCP4 | YHI9 | FSH1 | DAL2 | CFF1

Review For