Reference: Singh GP, et al. (2005) Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy. Anal Chem 77(8):2564-8

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Abstract


Living cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the Raman spectra of optically trapped Saccharomyces cerevisiae yeast cell under normal, heat-treated, and hyperosmotic stress conditions. It is shown that when glucose was used to exert hyperosmotic stress, two chemical substances-glycerol and ethanol-can be monitored in real time in a single cell.

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Journal Article | Research Support, Non-U.S. Gov't
Authors
Singh GP, Creely CM, Volpe G, Grötsch H, Petrov D
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