Reference: Börner GV, et al. (2004) Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis. Cell 117(1):29-45

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Abstract


Yeast mutants lacking meiotic proteins Zip1, Zip2, Zip3, Mer3, and/or Msh5 (ZMMs) were analyzed for recombination, synaptonemal complex (SC), and meiotic progression. At 33 degrees C, recombination-initiating double-strand breaks (DSBs) and noncrossover products (NCRs) form normally while formation of single-end invasion strand exchange intermediates (SEIs), double Holliday junctions, crossover products (CRs), and SC are coordinately defective. Thus, during wild-type meiosis, recombinational interactions are differentiated into CR and NCR types very early, prior to onset of stable strand exchange and independent of SC. By implication, crossover interference does not require SC formation. We suggest that SC formation may require interference. Subsequently, CR-designated DSBs undergo a tightly coupled, ZMM-promoted transition that yields SEI-containing recombination complexes embedded in patches of SC. zmm mutant phenotypes differ strikingly at 33 degrees C and 23 degrees C, implicating higher temperature as a positive effector of recombination and identifying a checkpoint that monitors local CR-specific events, not SC formation, at late leptotene.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Börner GV, Kleckner N, Hunter N
Primary Lit For
ZIP2 | HFM1 | MSH5 | ZIP1 | CST9 | Synapsis initiation complex | Synaptonemal complex

Gene Ontology Annotations 5 entries for 5 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
MSH5involved inreciprocal meiotic recombinationIMPSGD2013-08-07
ZIP1involved inreciprocal meiotic recombinationIMPSGD2013-08-07
ZIP2involved inreciprocal meiotic recombinationIMPSGD2013-08-07
HFM1involved inreciprocal meiotic recombinationIMPSGD2013-08-07
CST9involved inreciprocal meiotic recombinationIMPSGD2013-08-07
Showing 1 to 5 of 5 entries

Phenotype Annotations 4 entries for 4 genes


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
HFM1protein/peptide distribution: abnormal
Reporter: Zip1p
classical geneticsnull
Allele: hfm1-Δ
SK1
MSH5protein/peptide distribution: abnormal
Reporter: Zip1p
classical geneticsnull
Allele: msh5-Δ
SK1
ZIP2protein/peptide distribution: abnormal
Reporter: Zip1p
classical geneticsnull
Allele: zip2-Δ
SK1
CST9protein/peptide distribution: abnormal
Reporter: Zip1p
classical geneticsnull
Allele: cst9-Δ
SK1
Showing 1 to 4 of 4 entries