It was desired to study a simplified method of cellulase production using solid-state fermentation for its potential to be used on-site as part of a cellulose to ethanol conversion process, in lieu of expensive and energy intensive commercial enzyme preparations. Crude unprocessed cellulase extracts were produced by solid-state fermentation of Trichoderma reesei on ground wheat straw. While cellulase yields were not high they were sufficient to produce ethanol from wheat straw in simultaneous saccharification and fermentation with Saccharomyces cerevisiae. As little as an additional 5% of the material converted to ethanol may be employed for cellulase production suggesting an inordinate quantity of additional substrate would not be required. These findings suggest a simplified crude cellulase process at the site of ethanol production using a common lignocellulosic substrate may be employed in lieu of commercial enzyme preparations.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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