By pairwise crosses, 75 different fatty acid requiring mutants of Saccharomyces cerevisiae were subjected to complementation analysis. From the data obtained, a linear complementation map was constructed comprising nine different complementation groups. Eight of these groups had a polar complementation pattern. They failed to complement not only with members of their own but also with those of one or several other complementation groups. In all crosses investigated, genetic complementation was paralleled by the restoration of fatty acid synthetase activity in the heterozygous diploid. Two component enzyme activities of the fatty acid synthetase complex were studied with representatives of five different complementation groups. Hereby, four different activity patterns have been observed. It is concluded that at least these complementation groups represent different cistrons of the fatty acid synthetase system, although intracistronic complementation cannot be excluded for other groups, so far. Qualitatively, the mutants of eight different complementation groups are susceptible to mutual, UV-induced, mitotic recombination. This finding, together with the pleiotropy observed in complementation and enzyme defects, suggests that several fas-genes are linked and coordinatedly transcribed.

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Journal Article

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Schweizer E, Kühn L, Castorph H

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