The exocyst consists of eight rod-shaped subunits that align in a side-by-side manner to tether secretory vesicles to the plasma membrane in preparation for fusion. Two subunits, Sec3p and Exo70p, localize to exocytic sites by an actin-independent pathway, whereas the other six ride on vesicles along actin cables. Here, we demonstrate that three of the four domains of Exo70p are essential for growth. The remaining domain, domain C, is not essential but when deleted, it leads to synthetic lethality with many secretory mutations, defects in exocyst assembly of exocyst components Sec5p and Sec6p, and loss of actin-independent localization. This is analogous to a deletion of the amino-terminal domain of Sec3p, which prevents an interaction with Cdc42p or Rho1p and blocks its actin-independent localization. The two mutations are synthetically lethal, even in the presence of high copy number suppressors that can bypass complete deletions of either single gene. Although domain C binds Rho3p, loss of the Exo70p-Rho3p interaction does not account for the synthetic lethal interactions or the exocyst assembly defects. The results suggest that either Exo70p or Sec3p must associate with the plasma membrane for the exocyst to function as a vesicle tether.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| SEC3 | involved in | exocyst localization | IGI with EXO70 | SGD | 2013-08-07 | |
| EXO70 | involved in | exocyst assembly | IMP | SGD | 2013-08-07 | |
| EXO70 | involved in | exocyst localization | IGI with SEC3 | SGD | 2013-08-07 | |
| EXO70 | located in | cellular bud tip | IDA | SGD | 2013-08-07 | |
| EXO70 | located in | cellular bud neck | IDA | SGD | 2013-08-07 |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
|---|
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| EXO70 | SEC8 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| EXO84 | SEC6 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| RHO3 | EXO70 | Reconstituted Complex | manually curated | Bait-Hit | No Modification | |
| SEC15 | EXO70 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| SEC3 | EXO70 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| SEC5 | SEC8 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| SEC5 | SEC10 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| SEC5 | EXO70 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| SEC6 | SEC15 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| SEC6 | SEC8 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |