Reference: Zhang Y, et al. (2008) Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesis. Mol Cell Biol 28(18):5569-82

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Abstract


In a forward genetic screen for interaction with mitochondrial iron carrier proteins in Saccharomyces cerevisiae, a hypomorphic mutation of the essential DRE2 gene was found to confer lethality when combined with Delta mrs3 and Delta mrs4. The dre2 mutant or Dre2-depleted cells were deficient in cytosolic Fe/S cluster protein activities while maintaining mitochondrial Fe/S clusters. The Dre2 amino acid sequence was evolutionarily conserved, and cysteine motifs (CX(2)CXC and twin CX(2)C) in human and yeast proteins were perfectly aligned. The human Dre2 homolog (implicated in blocking apoptosis and called CIAPIN1 or anamorsin) was able to complement the nonviability of a Deltadre2 deletion strain. The Dre2 protein with triple hemagglutinin tag was located in the cytoplasm and in the mitochondrial intermembrane space. Yeast Dre2 overexpressed and purified from bacteria was brown and exhibited signature absorption and electron paramagnetic resonance spectra, indicating the presence of both [2Fe-2S] and [4Fe-4S] clusters. Thus, Dre2 is an essential conserved Fe/S cluster protein implicated in extramitochondrial Fe/S cluster assembly, similar to other components of the so-called CIA (cytoplasmic Fe/S cluster assembly) pathway although partially localized to the mitochondrial intermembrane space.

Reference Type
Journal Article | Research Support, N.I.H., Extramural | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, Non-P.H.S.
Authors
Zhang Y, Lyver ER, Nakamaru-Ogiso E, Yoon H, Amutha B, Lee DW, Bi E, Ohnishi T, Daldal F, Pain D, ... Show all
Primary Lit For
LEU1 | RPS2 | DRE2 | RPL25 | TAH18-DRE2 complex
Additional Lit For
MRS4 | MRS3 | dre2-137 | Gal-Dre2

Gene Ontology Annotations 3 entries for 1 gene


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
DRE2involved iniron-sulfur cluster assemblyIMPSGD2013-08-07
DRE2located incytosolIDASGD2013-08-07
DRE2located inmitochondrial intermembrane spaceIDASGD2013-08-07
Showing 1 to 3 of 3 entries

Phenotype Annotations 5 entries for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
DRE2protein activity: decreased
Reporter: IRP1

human cytosolic aconitase

classical geneticsrepressible
Allele: Gal-Dre2

DRE2 under Gal-inducible promoter

S288CMedia: media lacking galactose
DRE2protein activity: decreased
Reporter: Leu1p

pRS426-Leu1-His6

classical geneticsrepressible
Allele: Gal-Dre2

DRE2 under Gal-inducible promoter

S288CMedia: media lacking galactose
DRE2protein/peptide distribution: abnormal
Reporter: Rpl25p-eGFP

60S ribosomal subunit reporter

classical geneticsrepressible
Allele: Gal-Dre2

DRE2 under Gal-inducible promoter

S288CDetails: nuclear retention of ribosomal protein Rpl25p
DRE2utilization of iron source: decreased
classical geneticsreduction of function
Allele: dre2-137

K26stop

Other
DRE2vegetative growth: decreased
classical geneticsreduction of function
Allele: dre2-137

K26stop

OtherDetails: very slow growth rate
Showing 1 to 5 of 5 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 0 entries for 0 genes

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InteractorInteractorAssayAnnotationActionModification
No physical interaction data for Zhang Y, et al. (2008)
Showing 0 to 0 of 0 entries