Tekamp PA, et al. (1980)

Abstract

A method is described for the isolation of a yeast chromatin fraction highly enriched in ribosomal DNA sequences. In the presence of exogenous yeast RNA polymerase III, this purified chromatin actively synthesizes a set of 5 S ribosomal RNAs all of which have 5'-sequences identical with mature 5 S RNA but which end with a variable number (up to 10) of additional residues at the 3'-terminus. These extra nucleotides are precisely removed by a processing nuclease found in the chromatin supernatant fraction.

PMID: 6251092
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Reference Type: Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors: Tekamp PA, Garcea RL, Rutter WJ
Primary Lit For: DNA-directed RNA polymerase III complex
Additional Lit For: RDN5-1 | RDN5-2 | RDN5-3 | RDN5-4 | RDN5-5 | RDN5-6
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