Reference: Nakamura Y, et al. (2007)
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Abstract
The non-ATPase subunit Nas6, which is the human orthologue of gankyrin, was co-expressed with the C-terminal domain of the ATPase subunit Rpt3 of the yeast 26S proteasome in Escherichia coli, purified to near-homogeneity and crystallized using the hanging-drop vapour-diffusion method. The protein crystallized in space group P2(1), with unit-cell parameters a = 60.38, b = 100.22, c = 72.20 A, beta = 94.70 degrees and with three Nas6-Rpt3C molecules per asymmetric unit. The crystal diffracted to beyond 2.2 A resolution using synchrotron radiation.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't
- Authors
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Nakamura Y,
Umehara T,
Tanaka A,
Horikoshi M,
Padmanabhan B,
Yokoyama S
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- RPT3 | NAS6
Gene Ontology Annotations
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| Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
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Annotation Extension |
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Method |
Source |
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Gene |
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Reference |
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| Evidence ID |
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Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
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|
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
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| RPT3 | NAS6 | Co-crystal Structure | manually curated | Hit-Bait | No Modification |
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| Complement ID |
Locus ID |
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Species |
Gene ID |
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Direction |
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Reference |