Sixty-two alleles of the histidine-1 (his1) gene were tested for complementation. The 44 complementing mutants fell into 31 complementation groups which were used to construct a complex complementation map with 18 complementation units. Cluster analysis of the complementation map by either visual inspection or the computer method of Gillie and Peto (1969) shows two very definite clusters.The molecular weight estimate of the his1 enzyme, phosphoribosyl adenosine triphosphate: pyrophosphate phosphoribosyltransferase, is 1.8 . 10(5) by sucrose density gradient analysis and 2.4 . 10(5) by Sephadex gel chromatography. Correlating the length of the his1 gene to the molecular weight of the enzyme indicates that this enzyme is composed of 6 subunits, as is the analogous enzyme in Salmonella typhimurium.A model of the subunit and tertiary and quaternary structure of the enzyme has been developed from consideration of the genetic and complementation data, the distribution of the various mutant types within the gene, and the biochemical properties of the enzyme encoded by the his1 gene.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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