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Reference: Kinclova-Zimmermannova O, et al. (2006) The Na+,K+/H+ -antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae. FEMS Yeast Res 6(5):792-800

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Abstract

There are three different sodium transport systems (Ena1-4p, Nha1p, Nhx1p) in Saccharomyces cerevisiae. The effect of their absence on the tolerance to alkali-metal cations and on the membrane potential was studied. All three sodium transporters were found to participate in the maintenance of Na+, Li+, K+ and Cs+ homeostasis. Measurements of the distribution of a fluorescent potentiometric probe (diS-C3(3) assay) in cell suspensions showed that the lack of all three transporters depolarizes the plasma membrane. The overexpression of the Na+,K+/H+ antiporter Nha1 resulted in the hyperpolarization of the plasma membrane and consequently increased the sensitivity to Cs+, Tl+ and hygromycin B. This is the first evidence that the activity of a Na+,K+/H+ antiporter could play a role in the homeostatic regulation of the plasma membrane potential in yeast cells.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Kinclova-Zimmermannova O, Gaskova D, Sychrova H
Primary Lit For
ENA1 | NHX1 | NHA1

Phenotype Annotations 7 entries for 3 genes

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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
ENA1metal resistance: decreased
classical geneticsnull
Allele: ena1-Δ		W303200 mM sodium chloride
ENA1metal resistance: decreased
classical geneticsnull
Allele: ena1-Δ		W30310 mM lithium chloride
ENA1metal resistance: decreased
classical geneticsnull
Allele: ena1-Δ		W303800 mM KCl potassium(1+)
ENA1metal resistance: decreased
classical geneticsnull
Allele: ena1-Δ		W30340 mM CsCl caesium(1+)
NHX1viable
classical geneticsnull
Allele: nhx1-Δ		Other
NHA1viable
classical geneticsnull
Allele: nha1-Δ		Other
ENA1viable
classical geneticsnull
Allele: ena1-Δ		Other
Showing 1 to 7 of 7 entries