The antifungal activity of polygodial against Saccharomyces cerevisiae involves multifunctions. Polygodial first acts as a surface-active agent (surfactant) and then becomes involved in biochemical processes. The ability to form a pyrrole derivative with a primary amine group of phosphatidylethanolamine (PE) and phosphatidylserine (PS) in the outer monolayer of the plasma membrane is likely, in part, an initial step in the antifungal action of polygodial. In the lipid fraction derived from cells treated with polygodial, no PE and PS were detected, indicating a disturbance in the balance of the plasma membrane. The primary antifungal action of polygodial comes from its ability to act as a surfactant that nonspecifically disrupts the lipid-protein interface of integral proteins, denaturing their functioned conformation. Once polygodial enters the cytoplasm by destroying the membrane barrier, it reacts with L-cystein-containing cytoplasmic materials, such as a small molecule, glutathione, and a protein, alcohol dehydrogenase, to potentiate the antifungal action.

• PMID: 16122929
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Journal Article | Research Support, Non-U.S. Gov't

Authors

Fujita K, Kubo I

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