Reference: Van Hoof C, et al. (2005)
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Abstract
To elucidate the specific biological role of the yeast homologues of PTPA (phosphatase 2A phosphatase activator), Ypa1 and Ypa2 (where Ypa stands for yeast phosphatase activator), in the regulation of PP2A (protein phosphatase 2A), we investigated the physical interaction of both Ypa proteins with the catalytic subunit of the different yeast PP2A-like phosphatases. Ypa1 interacts specifically with Pph3, Sit4 and Ppg1, whereas Ypa2 binds to Pph21 and Pph22. The Ypa1 and Ypa2 proteins do not compete with Tap42 (PP2A associating protein) for binding to PP2A family members. The interaction of the Ypa proteins with the catalytic subunit of PP2A-like phosphatases is direct and independent of other regulatory subunits, implicating a specific function for the different PP2A-Ypa complexes. Strikingly, the interaction of Ypa2 with yeast PP2A is promoted by the presence of Ypa1, suggesting a positive role of Ypa1 in the regulation of PP2A association with other interacting proteins. As in the mammalian system, all yeast PP2A-like enzymes associate as an inactive complex with Yme (yeast methyl esterase). Ypa1 as well as Ypa2 can reactivate all these inactive complexes, except Pph22-Yme. Ypa1 is the most potent activator of PP2A activity, suggesting that there is no direct correlation between activation potential and binding capacity.
- Reference Type
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Comparative Study |
Journal Article |
Research Support, Non-U.S. Gov't
- Authors
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Van Hoof C,
Martens E,
Longin S,
Jordens J,
Stevens I,
Janssens V,
Goris J
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- RRD1 | RRD2
- PPH22 | PPH3 | PPE1 | PPH21 | SIT4 | PPG1 | TAP42 | TAP42-RRD2-PPH21 phosphatase complex | TAP42-RRD2-PPH22 phosphatase complex | TAP42-RRD1-SIT4 phosphatase complex
Gene Ontology Annotations
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Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
Aspect |
Annotation Extension |
Evidence |
Method |
Source |
Assigned On |
Reference |
Phenotype Annotations
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Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
Mutant Information |
Strain Background |
Chemical |
Details |
Reference |
Disease Annotations
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Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Disease Ontology Term |
Disease Ontology Term ID |
Qualifier |
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Source |
Assigned On |
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Reference |
Regulation Annotations
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Evidence ID |
Analyze ID |
Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
Regulator Type |
Direction |
Regulation Of |
Happens During |
Method |
Evidence |
Strain Background |
Reference |
Post-translational Modifications
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Site |
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Modification |
Modifier |
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Genetic Interactions
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Evidence ID |
Analyze ID |
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Interactor |
Interactor Systematic Name |
Interactor |
Interactor Systematic Name |
Allele |
Assay |
Annotation |
Action |
Phenotype |
SGA score |
P-value |
Source |
Reference |
Note |
Physical Interactions
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genes involved in the interaction.
| Interactor | Interactor | Assay | Annotation | Action | Modification |
| PPH21 | RRD2 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| PPH21 | TAP42 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| PPH22 | RRD2 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| PPH22 | TAP42 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| PPH3 | TAP42 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| RRD1 | PPH3 | Reconstituted Complex | manually curated | Bait-Hit | No Modification |
| RRD1 | SIT4 | Reconstituted Complex | manually curated | Bait-Hit | No Modification |
| RRD1 | SIT4 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| SIT4 | RRD2 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| SIT4 | TAP42 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
Functional Complementation Annotations
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Complement ID |
Locus ID |
Gene |
Species |
Gene ID |
Strain background |
Direction |
Details |
Source |
Reference |