Reference: Armache KJ, et al. (2005)
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Abstract
We determined the x-ray structure of the RNA polymerase (Pol) II subcomplex Rpb4/7 at 2.3 A resolution, combined it with a previous structure of the 10-subunit polymerase core, and refined an atomic model of the complete 12-subunit Pol II at 3.8-A resolution. Comparison of the complete Pol II structure with structures of the Pol II core and free Rpb4/7 shows that the core-Rpb4/7 interaction goes along with formation of an alpha-helix in the linker region of the largest Pol II subunit and with folding of the conserved Rpb7 tip loop. Details of the core-Rpb4/7 interface explain facilitated Rpb4/7 dissociation in a temperature-sensitive Pol II mutant and specific assembly of Pol I with its Rpb4/7 counterpart, A43/14. The refined atomic model of Pol II serves as the new reference structure for analysis of the transcription mechanism and enables structure solution of complexes of the complete enzyme with additional factors and nucleic acids by molecular replacement.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't
- Authors
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Armache KJ,
Mitterweger S,
Meinhart A,
Cramer P
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- RPB9 | RPB7 | RPB4 | RPC10 | RPB5 | RPO21 | RPB3 | RPO26 | RPB11 | RPB10 | RPB8 | ... Show allRPB2 | RPB4-RPB7 subcomplex Show fewer
Gene Ontology Annotations
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|
| RPB4-RPB7 subcomplex | located in | P-body | | BSR | ComplexPortal | 2014-10-16 |
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Reference |
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Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
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Happens During |
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| Evidence ID |
Analyze ID |
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Interactor |
Interactor Systematic Name |
Interactor |
Interactor Systematic Name |
Allele |
Assay |
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SGA score |
P-value |
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Note |
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
|---|
| RPB7 | RPB4 | Co-crystal Structure | manually curated | Hit-Bait | No Modification |
Functional Complementation Annotations
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| Complement ID |
Locus ID |
Gene |
Species |
Gene ID |
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Direction |
Details |
Source |
Reference |