Reference: Lo WS, et al. (2005)
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Abstract
Histone phosphorylation influences transcription, chromosome condensation, DNA repair and apoptosis. Previously, we showed that histone H3 Ser10 phosphorylation (pSer10) by the yeast Snf1 kinase regulates INO1 gene activation in part via Gcn5/SAGA complex-mediated Lys14 acetylation (acLys14). How such chromatin modification patterns develop is largely unexplored. Here we examine the mechanisms surrounding pSer10 at INO1, and at GAL1, which herein is identified as a new regulatory target of Snf1/pSer10. Snf1 behaves as a classic coactivator in its recruitment by DNA-bound activators, and in its role in modifying histones and recruiting TATA-binding protein (TBP). However, one important difference in Snf1 function in vivo at these promoters is that SAGA recruitment at INO1 requires histone phosphorylation via Snf1, whereas at GAL1, SAGA recruitment is independent of histone phosphorylation. In addition, the GAL1 activator physically interacts with both Snf1 and SAGA, whereas the INO1 activator interacts only with Snf1. Thus, at INO1, pSer10's role in recruiting SAGA may substitute for recruitment by DNA-bound activator. Our results emphasize that histone modifications share general functions between promoters, but also acquire distinct roles tailored for promoter-specific requirements.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't |
Research Support, U.S. Gov't, Non-P.H.S. |
Research Support, U.S. Gov't, P.H.S.
- Authors
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Lo WS,
Gamache ER,
Henry KW,
Yang D,
Pillus L,
Berger SL
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- HHT1 | GAL1 | HHT2 | SNF1 | SAGA complex
- INO1 | GCN5 | SPT15 | Snf1 protein kinase complex variant GAL83 | Snf1 protein kinase complex variant SIP2
Gene Ontology Annotations
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Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
Aspect |
Annotation Extension |
Evidence |
Method |
Source |
Assigned On |
Reference |
Phenotype Annotations
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Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
Mutant Information |
Strain Background |
Chemical |
Details |
Reference |
Disease Annotations
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Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Disease Ontology Term |
Disease Ontology Term ID |
Qualifier |
Evidence |
Method |
Source |
Assigned On |
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Reference |
Regulation Annotations
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further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, or SPELL.
Evidence ID |
Analyze ID |
Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
Regulator Type |
Direction |
Regulation Of |
Happens During |
Method |
Evidence |
Strain Background |
Reference |
Post-translational Modifications
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Site |
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Modification |
Modifier |
Source |
Reference |
Interaction Annotations
Genetic Interactions
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genes involved in the interaction.
Evidence ID |
Analyze ID |
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Interactor |
Interactor Systematic Name |
Interactor |
Interactor Systematic Name |
Allele |
Assay |
Annotation |
Action |
Phenotype |
SGA score |
P-value |
Source |
Reference |
Note |
Physical Interactions
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genes involved in the interaction.
| Interactor | Interactor | Assay | Annotation | Action | Modification |
| GAL83 | SNF1 | Affinity Capture-MS | manually curated | Hit-Bait | No Modification |
| INO2 | GAL4 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| SNF1 | SNF4 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification |
| SNF1 | SIP2 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification |
| SNF1 | GAL4 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
Functional Complementation Annotations
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Complement ID |
Locus ID |
Gene |
Species |
Gene ID |
Strain background |
Direction |
Details |
Source |
Reference |