Reference: Kang JY, et al. (2005) PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol. J Biol Chem 280(10):9489-97

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Abstract


Glycosylphosphatidylinositol (GPI) is a glycolipid that anchors many proteins to the eukaryotic cell surface. The biosynthetic pathway of GPI is mediated by sequential additions of sugars and other components to phosphatidylinositol. Four mannoses in the GPI are transferred from dolichol-phosphate-mannose (Dol-P-Man) and are linked through different glycosidic linkages. Therefore, four Dol-P-Man-dependent mannosyltransferases, GPI-MT-I, -MT-II, -MT-III, and -MT-IV for the first, second, third, and fourth mannoses, respectively, are required for generation of GPI. GPI-MT-I (PIG-M), GPI-MT-III (PIG-B), and GPI-MT-IV (SMP3) were previously reported, but GPI-MT-II remains to be identified. Here we report the cloning of PIG-V involved in transferring the second mannose in the GPI anchor. Human PIG-V encodes a 493-amino acid, endoplasmic reticulum (ER) resident protein with eight putative transmembrane regions. Saccharomyces cerevisiae protein encoded in open reading frame YBR004c, which we termed GPI18, has 25% amino acid identity to human PIG-V. Viability of the yeast gpi18 deletion mutant was restored by human PIG-V cDNA. PIG-V has two functionally important conserved regions facing the ER lumen. Taken together, we suggest that PIG-V is the second mannosyltransferase in GPI anchor biosynthesis.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Kang JY, Hong Y, Ashida H, Shishioh N, Murakami Y, Morita YS, Maeda Y, Kinoshita T
Primary Lit For
GPI18
Additional Lit For
Glycosylphosphatidylinositol-mannosyltransferase II complex

Gene Ontology Annotations 2 entries for 1 gene


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
GPI18involved inGPI anchor biosynthetic processIGI with BAA91196SGD2013-08-07
GPI18enablesmannosyltransferase activityIGI with BAA91196SGD2013-08-07
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Phenotype Annotations 1 entry for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
GPI18inviable
classical geneticsnull
Allele: gpi18-Δ
S288C
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Functional Complementation Annotations 1 entry for 1 complement


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GeneSpeciesGene IDStrain backgroundDirectionDetailsSource
GPI18Homo sapiensHGNC:26031S288Cother complements yeastP-POD
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