Reference: Saito K, et al. (2004) Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae. Mol Biol Cell 15(7):3418-32

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Abstract


Cdc50p, a transmembrane protein localized to the late endosome, is required for polarized cell growth in yeast. Genetic studies suggest that CDC50 performs a function similar to DRS2, which encodes a P-type ATPase of the aminophospholipid translocase (APT) subfamily. At low temperatures, drs2Delta mutant cells exhibited depolarization of cortical actin patches and mislocalization of polarity regulators, such as Bni1p and Gic1p, in a manner similar to the cdc50Delta mutant. Both Cdc50p and Drs2p were localized to the trans-Golgi network and late endosome. Cdc50p was coimmunoprecipitated with Drs2p from membrane protein extracts. In cdc50Delta mutant cells, Drs2p resided on the endoplasmic reticulum (ER), whereas Cdc50p was found on the ER membrane in drs2Delta cells, suggesting that the association on the ER membrane is required for transport of the Cdc50p-Drs2p complex to the trans-Golgi network. Lem3/Ros3p, a homolog of Cdc50p, was coimmunoprecipitated with another APT, Dnf1p; Lem3p was required for exit of Dnf1p out of the ER. Both Cdc50p-Drs2p and Lem3p-Dnf1p were confined to the plasma membrane upon blockade of endocytosis, suggesting that these proteins cycle between the exocytic and endocytic pathways, likely performing redundant functions. Thus, phospholipid asymmetry plays an important role in the establishment of cell polarity; the Cdc50p/Lem3p family likely constitute potential subunits specific to unique P-type ATPases of the APT subfamily.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Saito K, Fujimura-Kamada K, Furuta N, Kato U, Umeda M, Tanaka K
Primary Lit For
DRS2 | NEO1 | DNF1 | LEM3 | CDC50 | DRS2-CDC50 P4-ATPase complex | DNF1-LEM3 P4-ATPase complex
Additional Lit For
GIC1 | ACT1 | YNR048W | BNI1

Gene Ontology Annotations 10 entries for 5 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
DRS2-CDC50 P4-ATPase complexis active intrans-Golgi networkIDASGD2024-08-21
DRS2located intrans-Golgi networkIDASGD2013-08-07
DRS2part ofCdc50p-Drs2p complexIPI with CDC50SGD2014-12-03
CDC50located intrans-Golgi networkIDASGD2013-08-07
CDC50involved inphospholipid translocationIGI with LEM3SGD2013-08-07
CDC50contributes toaminophospholipid flippase activityIGI with LEM3SGD2019-06-21
CDC50contributes toaminophospholipid flippase activityIGI with VRP1SGD2019-06-21
CDC50part ofCdc50p-Drs2p complexIPI with DRS2SGD2014-12-03
DNF1part ofphospholipid-translocating ATPase complexIPI with LEM3SGD2014-12-05
LEM3part ofphospholipid-translocating ATPase complexIPI with DNF1SGD2014-12-05
Showing 1 to 10 of 10 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 4 entries for 4 genes

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InteractorInteractorAssayAnnotationActionModification
DNF1LEM3Affinity Capture-Westernmanually curatedBait-HitNo Modification
DRS2CDC50Affinity Capture-Westernmanually curatedBait-HitNo Modification
DRS2CDC50Affinity Capture-Westernmanually curatedHit-BaitNo Modification
DRS2LEM3Affinity Capture-Westernmanually curatedBait-HitNo Modification
Showing 1 to 4 of 4 entries