Cdc50p, a transmembrane protein localized to the late endosome, is required for polarized cell growth in yeast. Genetic studies suggest that CDC50 performs a function similar to DRS2, which encodes a P-type ATPase of the aminophospholipid translocase (APT) subfamily. At low temperatures, drs2Delta mutant cells exhibited depolarization of cortical actin patches and mislocalization of polarity regulators, such as Bni1p and Gic1p, in a manner similar to the cdc50Delta mutant. Both Cdc50p and Drs2p were localized to the trans-Golgi network and late endosome. Cdc50p was coimmunoprecipitated with Drs2p from membrane protein extracts. In cdc50Delta mutant cells, Drs2p resided on the endoplasmic reticulum (ER), whereas Cdc50p was found on the ER membrane in drs2Delta cells, suggesting that the association on the ER membrane is required for transport of the Cdc50p-Drs2p complex to the trans-Golgi network. Lem3/Ros3p, a homolog of Cdc50p, was coimmunoprecipitated with another APT, Dnf1p; Lem3p was required for exit of Dnf1p out of the ER. Both Cdc50p-Drs2p and Lem3p-Dnf1p were confined to the plasma membrane upon blockade of endocytosis, suggesting that these proteins cycle between the exocytic and endocytic pathways, likely performing redundant functions. Thus, phospholipid asymmetry plays an important role in the establishment of cell polarity; the Cdc50p/Lem3p family likely constitute potential subunits specific to unique P-type ATPases of the APT subfamily.
Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.
Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
---|---|---|---|---|---|---|
DRS2-CDC50 P4-ATPase complex | is active in | trans-Golgi network | IDA | SGD | 2024-08-21 | |
DRS2 | located in | trans-Golgi network | IDA | SGD | 2013-08-07 | |
DRS2 | part of | Cdc50p-Drs2p complex | IPI with CDC50 | SGD | 2014-12-03 | |
CDC50 | located in | trans-Golgi network | IDA | SGD | 2013-08-07 | |
CDC50 | involved in | phospholipid translocation | IGI with LEM3 | SGD | 2013-08-07 | |
CDC50 | contributes to | aminophospholipid flippase activity | IGI with LEM3 | SGD | 2019-06-21 | |
CDC50 | contributes to | aminophospholipid flippase activity | IGI with VRP1 | SGD | 2019-06-21 | |
CDC50 | part of | Cdc50p-Drs2p complex | IPI with DRS2 | SGD | 2014-12-03 | |
DNF1 | part of | phospholipid-translocating ATPase complex | IPI with LEM3 | SGD | 2014-12-05 | |
LEM3 | part of | phospholipid-translocating ATPase complex | IPI with DNF1 | SGD | 2014-12-05 |
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
---|
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
Interactor | Interactor | Assay | Annotation | Action | Modification | |
---|---|---|---|---|---|---|
DNF1 | LEM3 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
DRS2 | CDC50 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
DRS2 | CDC50 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
DRS2 | LEM3 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |