In vitro transcription of templates containing deletion-substitution mutations has localized two essential promoter elements of the 5 S rRNA gene of Saccharomyces cerevisiae. A promoter element spanning the start site of transcription extends from -14 to +8, and a short internal control region (ICR) extends from +81 to +94. Changes in spacing between these elements by more than a few base pairs significantly reduce transcription. The site of RNA polymerase III transcription factor A (TFIIIA) binding, mapped by determination of the G residues that are protected from methylation on exposure of the TFIIIA.5 S DNA complex to dimethyl sulfate, is coincident with the ICR. Incorporation of TFIIIC into the TFIIIA.5 S rRNA gene complex protects additional G residues 5' and 3' of the ICR from methylation.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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