Reference: Hollingsworth MJ and Martin NC (1986) RNase P activity in the mitochondria of Saccharomyces cerevisiae depends on both mitochondrion and nucleus-encoded components. Mol Cell Biol 6(4):1058-64

Reference Help

Abstract


A requisite step in the biosynthesis of tRNA is the removal of 5' leader sequences from tRNA precursors. We have detected an RNase P activity in yeast mitochondrial extracts that can carry out this reaction on a homologous precursor tRNA. This mitochondrial RNase P was sensitive to both micrococcal nuclease and protease, demonstrating that it requires both a nucleic acid and protein for activity. The presence of RNase P activity in vitro directly correlated with the presence of a locus on yeast mitochondrial DNA previously shown by genetic and biochemical studies to be required for tRNA maturation. The product of the locus, the 9S RNA, and this newly described mitochondrial RNase P activity cofractionated, providing further evidence that the 9S RNA is the RNA component of yeast mitochondrial RNase P.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Hollingsworth MJ, Martin NC
Primary Lit For
RPM1 | RPM2 | Mitochondrial ribonuclease P complex

Gene Ontology Annotations 3 entries for 2 genes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
RPM2part ofmitochondrial ribonuclease P complexIDASGD2013-08-07
RPM1involved intRNA 5'-leader removalIDASGD2007-03-01
RPM1part ofmitochondrial ribonuclease P complexIDASGD2007-03-01
Showing 1 to 3 of 3 entries