Spore formation in Saccharomyces cerevisiae occurs via the de novo synthesis of the prospore membrane during the second meiotic division. Prospore membrane formation is triggered by assembly of a membrane-organizing center, the meiotic outer plaque (MOP), on the cytoplasmic face of the spindle pole body (SPB) during meiosis. We report here the identification of two new components of the MOP, Ady4p and Spo74p. Ady4p and Spo74p interact with known proteins of the MOP and are localized to the outer plaque of the SPB during meiosis II. MOP assembly and prospore membrane formation are abolished in spo74Delta/spo74Delta cells and occur aberrantly in ady4Delta/ady4Delta cells. Spo74p and the MOP component Mpc70p are mutually dependent for recruitment to SPBs during meiosis. In contrast, both Ady4p and Spo74p are present at SPBs, albeit at reduced levels, in cells that lack the MOP component Mpc54p. Our findings suggest a model for the assembled MOP in which Mpc54p, Mpc70p, and Spo74p make up a core structural unit of the scaffold that initiates synthesis of the prospore membrane, and Ady4p is an auxiliary component that stabilizes the plaque.
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Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
---|---|---|---|---|---|---|
SPO74 | located in | meiotic spindle pole body | IMP | SGD | 2016-06-29 | |
SPO74 | enables | structural molecule activity | IDA | SGD | 2013-08-07 | |
SPO74 | enables | structural molecule activity | IMP | SGD | 2013-08-07 | |
SPO74 | located in | meiotic spindle pole body | IDA | SGD | 2016-06-29 | |
ADY4 | located in | meiotic spindle pole body | IMP | SGD | 2016-06-29 | |
ADY4 | enables | structural molecule activity | IMP | SGD | 2013-08-07 | |
ADY4 | located in | meiotic spindle pole body | IDA | SGD | 2016-06-29 | |
ADY4 | enables | structural molecule activity | IDA | SGD | 2013-08-07 | |
SPO21 | enables | structural molecule activity | IDA | SGD | 2013-08-07 | |
SPO21 | enables | structural molecule activity | IMP | SGD | 2013-08-07 |
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Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
---|---|---|---|---|---|---|
SPO74 | sporulation: abnormal | heterozygous diploid | null Allele: spo74-Δ | SK1 | Details: accumulates non-sister dyads | |
ADY4 | sporulation: abnormal | homozygous diploid | null Allele: ady4-Δ | SK1 | Details: accumulates random dyads; meiotic outer plaque assembly and prospore membrane formation are aberrant | |
SPO74 | sporulation: absent | homozygous diploid | null Allele: spo74-Δ | SK1 | Details: meiotic outer plaque assembly and prospore membrane formation are abolished |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Interactor | Interactor | Assay | Annotation | Action | Modification | |
---|---|---|---|---|---|---|
ADY4 | CNM67 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
ADY4 | SPO21 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
ADY4 | NUD1 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
SPO21 | MPC54 | Two-hybrid | manually curated | Bait-Hit | No Modification | |
SPO74 | SPO21 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
SPO74 | SPO74 | Two-hybrid | manually curated | Hit-Bait | No Modification |