Reference: Lewis MJ and Pelham HR (2002) A new yeast endosomal SNARE related to mammalian syntaxin 8. Traffic 3(12):922-9

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Abstract


We report the identification of a yeast SNARE that has escaped notice because of an apparent error in the genome sequence and because it is functionally redundant. It is encoded by an extended version of ORF YAL014c, and since its SNARE motif is related to mammalian syntaxin 8 we term the gene SYN8. Syn8p is in endosomes. Co-precipitation indicates a set of complexes containing Pep12p, Vti1p, either Syn8p or Tlg1p and either Snc1p or Ykt6p. Analysis of growth and trafficking defects demonstrates that in the absence of Tlg1p, Syn8p is required for Pep12p function. Conversely, when Tlg1p is present, Syn8p can be removed without loss of Pep12p function, or induction of any other obvious trafficking defect. Syn8p thus appears to be a functional homolog of mammalian syntaxin 8, but Tlg1p can, amongst other roles, provide an equivalent function.

Reference Type
Journal Article
Authors
Lewis MJ, Pelham HR
Primary Lit For
SYN8 | YKT6 | PEP12 | TLG1 | Endosomal SNARE complex PEP12-VTI1-SYN8-YKT6 | Endosomal SNARE complex PEP12-VTI1-TLG1-YKT6 | Endosomal SNARE complex PEP12-VTI1-TLG1-SNC1 | Endosomal SNARE complex PEP12-VTI1-SYN8-SNC1 | Endosomal SNARE complex PEP12-VTI1-SYN8-SNC2 | Endosomal SNARE complex PEP12-VTI1-TLG1-SNC2
Additional Lit For
SNC1 | VTI1

Gene Ontology Annotations


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Gene/Complex Qualifier Gene Ontology Term Aspect Annotation Extension Evidence Method Source Assigned On Reference

Phenotype Annotations 1 entry for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
SYN8vacuolar transport: abnormal
Reporter: carboxypeptidase Y
classical geneticsnull
Allele: syn8-Δ
OtherDetails: minor, but reproducible missorting defect
Showing 1 to 1 of 1 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 7 entries for 6 genes

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InteractorInteractorAssayAnnotationActionModification
SNC1PEP12Affinity Capture-Westernmanually curatedBait-HitNo Modification
SYN8PEP12Affinity Capture-Westernmanually curatedBait-HitNo Modification
SYN8SNC1Affinity Capture-Westernmanually curatedBait-HitNo Modification
SYN8VTI1Affinity Capture-Westernmanually curatedBait-HitNo Modification
SYN8YKT6Affinity Capture-Westernmanually curatedBait-HitNo Modification
TLG1PEP12Affinity Capture-Westernmanually curatedBait-HitNo Modification
YKT6PEP12Affinity Capture-Westernmanually curatedHit-BaitNo Modification
Showing 1 to 7 of 7 entries