We report the identification of a yeast SNARE that has escaped notice because of an apparent error in the genome sequence and because it is functionally redundant. It is encoded by an extended version of ORF YAL014c, and since its SNARE motif is related to mammalian syntaxin 8 we term the gene SYN8. Syn8p is in endosomes. Co-precipitation indicates a set of complexes containing Pep12p, Vti1p, either Syn8p or Tlg1p and either Snc1p or Ykt6p. Analysis of growth and trafficking defects demonstrates that in the absence of Tlg1p, Syn8p is required for Pep12p function. Conversely, when Tlg1p is present, Syn8p can be removed without loss of Pep12p function, or induction of any other obvious trafficking defect. Syn8p thus appears to be a functional homolog of mammalian syntaxin 8, but Tlg1p can, amongst other roles, provide an equivalent function.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| SNC1 | PEP12 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| SYN8 | PEP12 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| SYN8 | SNC1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| SYN8 | VTI1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| SYN8 | YKT6 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| TLG1 | PEP12 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| YKT6 | PEP12 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |