Reference: Dixon C, et al. (2003) Overproduction of polypeptides corresponding to the amino terminus of the F-box proteins Cdc4p and Met30p inhibits ubiquitin ligase activities of their SCF complexes. Eukaryot Cell 2(1):123-33

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Abstract


Ubiquitin ligases direct the transfer of ubiquitin onto substrate proteins and thus target the substrate for proteasome-dependent degradation. SCF complexes are a family of ubiquitin ligases composed of a common core of components and a variable component called an F-box protein that defines substrate specificity. Distinct SCF complexes, defined by a particular F-box protein, target different substrate proteins for degradation. Although a few have been identified to be involved in important biological pathways, such as the cell division cycle and coordinating cellular responses to changes in environmental conditions, the role of the overwhelming majority of F-box proteins is not clear. Creating inhibitors that will block the in vivo activities of specific SCF ubiquitin ligases may provide identification of substrates of these uncharacterized F-box proteins. Using Saccharomyces cerevisiae as a model system, we demonstrate that overproduction of polypeptides corresponding to the amino terminus of the F-box proteins Cdc4p and Met30p results in specific inhibition of their SCF complexes. Analyses of mutant amino-terminal alleles demonstrate that the interaction of these polypeptides with their full-length counterparts is an important step in the inhibitory process. These results suggest a common means to inhibit specific SCF complexes in vivo.

Reference Type
Journal Article | Research Support, U.S. Gov't, Non-P.H.S.
Authors
Dixon C, Brunson LE, Roy MM, Smothers D, Sehorn MG, Mathias N
Primary Lit For
MET30 | CDC4 | SCF-Cdc4 ubiquitin ligase complex | SCF-MET30 E3 ubiquitin ligase complex

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 4 entries for 3 genes

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InteractorInteractorAssayAnnotationActionModification
CDC4CDC4Affinity Capture-Westernmanually curatedHit-BaitNo Modification
CDC4SKP1Two-hybridmanually curatedHit-BaitNo Modification
CDC4CDC4Two-hybridmanually curatedHit-BaitNo Modification
MET30SKP1Two-hybridmanually curatedHit-BaitNo Modification
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