Reference: Sterner DE, et al. (2002) SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription. Proc Natl Acad Sci U S A 99(18):11622-7

Reference Help

Abstract


Spt-Ada-Gcn5 acetyltransferase (SAGA) is a previously described histone acetyltransferase/transcriptional coactivator complex in yeast. At promoters of certain genes (HIS3 and TRP3), SAGA has an inhibitory function involving a nonproductive TATA-binding protein interaction mediated by the Spt3 and Spt8 subunits. Related to this, Spt8-less SAGA is a major form of the complex under activating conditions for these genes. In the present study, we purify this activation-specific complex, called SALSA (SAGA altered, Spt8 absent). Besides lacking Spt8, SALSA contains Spt7 subunit that is truncated. Examining the role of this subunit, we find that C-terminally truncated SPT7 resulted in derepressed HIS3 transcription. Furthermore, when grown in rich media (repressing conditions), wild-type cells yielded predominantly SAGA, but Spt7 C-terminal truncations resulted primarily in a form of complex similar to SALSA. Thus, SALSA-like structure and activating function can be partially recapitulated in yeast by truncating the C terminus of Spt7. Overall, these results lead to a model that for a subset of promoters SAGA is inhibitory through Spt3, Spt8, and an Spt8-interacting subdomain of Spt7, whereas SALSA is a form of complex for positive transcriptional regulation. These data clarify a mechanism by which a transcriptional regulatory complex can switch between positive and negative modulation.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, Non-P.H.S. | Research Support, U.S. Gov't, P.H.S.
Authors
Sterner DE, Belotserkovskaya R, Berger SL
Primary Lit For
SPT7 | SPT3 | GCN5 | SPT8 | SAGA complex | SLIK (SAGA-like) complex
Additional Lit For
TRP3 | HIS3

Interaction Annotations


Genetic Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 18 entries for 11 genes

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

InteractorInteractorAssayAnnotationActionModification
ADA2HFI1Affinity Capture-Westernmanually curatedBait-HitNo Modification
ADA2GCN5Affinity Capture-Westernmanually curatedBait-HitNo Modification
ADA2SPT20Affinity Capture-Westernmanually curatedBait-HitNo Modification
ADA2SPT8Affinity Capture-Westernmanually curatedBait-HitNo Modification
ADA2TAF6Affinity Capture-Westernmanually curatedBait-HitNo Modification
NGG1SPT20Affinity Capture-Westernmanually curatedHit-BaitNo Modification
NGG1SPT3Affinity Capture-Westernmanually curatedHit-BaitNo Modification
NGG1SPT8Affinity Capture-Westernmanually curatedHit-BaitNo Modification
SPT20HFI1Affinity Capture-Westernmanually curatedBait-HitNo Modification
SPT3ADA2Affinity Capture-Westernmanually curatedHit-BaitNo Modification
Showing 1 to 10 of 18 entries