Reference: Sterner DE, et al. (2002)
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Abstract
Spt-Ada-Gcn5 acetyltransferase (SAGA) is a previously described histone acetyltransferase/transcriptional coactivator complex in yeast. At promoters of certain genes (HIS3 and TRP3), SAGA has an inhibitory function involving a nonproductive TATA-binding protein interaction mediated by the Spt3 and Spt8 subunits. Related to this, Spt8-less SAGA is a major form of the complex under activating conditions for these genes. In the present study, we purify this activation-specific complex, called SALSA (SAGA altered, Spt8 absent). Besides lacking Spt8, SALSA contains Spt7 subunit that is truncated. Examining the role of this subunit, we find that C-terminally truncated SPT7 resulted in derepressed HIS3 transcription. Furthermore, when grown in rich media (repressing conditions), wild-type cells yielded predominantly SAGA, but Spt7 C-terminal truncations resulted primarily in a form of complex similar to SALSA. Thus, SALSA-like structure and activating function can be partially recapitulated in yeast by truncating the C terminus of Spt7. Overall, these results lead to a model that for a subset of promoters SAGA is inhibitory through Spt3, Spt8, and an Spt8-interacting subdomain of Spt7, whereas SALSA is a form of complex for positive transcriptional regulation. These data clarify a mechanism by which a transcriptional regulatory complex can switch between positive and negative modulation.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't |
Research Support, U.S. Gov't, Non-P.H.S. |
Research Support, U.S. Gov't, P.H.S.
- Authors
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Sterner DE,
Belotserkovskaya R,
Berger SL
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- SPT7 | SPT3 | GCN5 | SPT8 | SAGA complex | SLIK (SAGA-like) complex
- TRP3 | HIS3
Gene Ontology Annotations
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Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
Aspect |
Annotation Extension |
Evidence |
Method |
Source |
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Reference |
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Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
Mutant Information |
Strain Background |
Chemical |
Details |
Reference |
Disease Annotations
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Analyze ID |
Gene |
Gene Systematic Name |
Disease Ontology Term |
Disease Ontology Term ID |
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Source |
Assigned On |
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Reference |
Regulation Annotations
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Evidence ID |
Analyze ID |
Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
Regulator Type |
Direction |
Regulation Of |
Happens During |
Method |
Evidence |
Strain Background |
Reference |
Post-translational Modifications
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Genetic Interactions
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Analyze ID |
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Interactor |
Interactor Systematic Name |
Interactor |
Interactor Systematic Name |
Allele |
Assay |
Annotation |
Action |
Phenotype |
SGA score |
P-value |
Source |
Reference |
Note |
Physical Interactions
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
| ADA2 | HFI1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| ADA2 | GCN5 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| ADA2 | SPT20 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| ADA2 | SPT8 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| ADA2 | TAF6 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| NGG1 | SPT20 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| NGG1 | SPT3 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| NGG1 | SPT8 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| SPT20 | HFI1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| SPT3 | ADA2 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
Functional Complementation Annotations
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Complement ID |
Locus ID |
Gene |
Species |
Gene ID |
Strain background |
Direction |
Details |
Source |
Reference |