Reference: Papamichos-Chronakis M, et al. (2002)
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Abstract
The yeast Cyc8 and Tup1 proteins form a corepressor complex that, when tethered to DNA, turns off transcription. Release of the Cyc8-Tup1 corepressor from a promoter has been considered as a prerequisite for subsequent transcriptional activation. Contrasting this, we demonstrate that Cyc8-Tup1 is continuously associated with target promoters under both repressive and inducing conditions. At the GAL1 promoter, Cyc8-Tup1 facilitates recruitment of SAGA (Spt-Ada-Gcn5-acetyltranferase) via Cti6, a PHD domain protein that physically links the Cyc8-Tup1 and SAGA complexes. Lack of functional corepressor renders GAL1 transcription largely independent of specific SAGA subunits. Thus, corepressor's release is not the mechanism of derepression; instead, it is the coactivator complex that alleviates Cyc8-Tup1-mediated repression under induction conditions.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't
- Authors
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Papamichos-Chronakis M,
Petrakis T,
Ktistaki E,
Topalidou I,
Tzamarias D
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- SPT3 | CYC8 | SUC2 | GAL1 | ANB1 | HSP26 | GCN5 | GAL4 | GAL80 | TUP1 | SPT20 | ... Show allCTI6 | SAGA complex Show fewer
- NGG1 | ADA2
Gene Ontology Annotations
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| Evidence ID |
Analyze ID |
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Reference |
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
|---|
| ADA2 | CTI6 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| CYC8 | CTI6 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| CYC8 | GCN5 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| CYC8 | CTI6 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
| GCN5 | CTI6 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| NGG1 | CTI6 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| SPT3 | CTI6 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
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| Complement ID |
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Species |
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Reference |