Reference: Meira LB, et al. (1995) 8-Methoxypsoralen photoinduced plasmid-chromosome recombination in Saccharomyces cerevisiae using a centromeric vector. Nucleic Acids Res 23(9):1614-20

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Abstract


The characterization of a new system to study the induction of plasmid-chromosome recombination is described. Single-stranded and double-stranded centromeric vectors bearing 8-methoxypsoralen photoinduced lesions were used to transform a wild-type yeast strain bearing the leu2-3,112 marker. Using the SSCP methodology and DNA sequencing, it was demonstrated that repair of the lesions in plasmid DNA was mainly due to conversion of the chromosomal allele to the plasmid DNA.

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Journal Article | Research Support, Non-U.S. Gov't
Authors
Meira LB, Henriques JA, Magaña-Schwencke N
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