The Prp19p-associated complex is essential for the yeast pre-mRNA splicing reaction. The complex consists of at least eight protein components, but is not tightly associated with spliceosomal snRNAs. By a combination of genetic and biochemical methods we previously identified four components of this complex, Ntc25p, Ntc85p, Ntc30p and Ntc20p, all of them being novel splicing factors. We have now identified three other components of the complex, Ntc90p, Ntc77p and Ntc31p. These three proteins were also associated with the spliceosome during the splicing reaction in the same manner as Prp19p, concurrently with or immediately after dissociation of U4 snRNA. Two-hybrid analysis revealed that none of these proteins interacted with Prp19p or Ntc25p, but all interacted with Ntc85p. An interaction network between the identified components of the Prp19p-associated complex is demonstrated. Biochemical analysis revealed that Ntc90p, Ntc31p, Ntc30p and Ntc20p form a subcomplex, which, through interacting with Ntc85p and Ntc77p, can associate with Prp19p and Ntc25p to form the Prp19p-associated complex. Genetic analysis suggests that Ntc31p, Ntc30p and Ntc20p may play roles in modulating the function of Ntc90p.
Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.
| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| SYF1 | part of | Prp19 complex | IDA | SGD | 2013-08-07 | |
| SYF1 | contributes to | first spliceosomal transesterification activity | IC with U2-type catalytic step 1 spliceosome | SGD | 2013-08-07 | |
| SYF1 | part of | U2-type catalytic step 1 spliceosome | IDA | SGD | 2013-08-07 | |
| SYF2 | contributes to | first spliceosomal transesterification activity | IC with U2-type catalytic step 1 spliceosome | SGD | 2013-08-07 | |
| SYF2 | part of | Prp19 complex | IDA | SGD | 2013-08-07 | |
| SYF2 | part of | U2-type catalytic step 1 spliceosome | IDA | SGD | 2013-08-07 | |
| CLF1 | part of | U2-type catalytic step 1 spliceosome | IDA | SGD | 2013-08-07 | |
| CLF1 | part of | Prp19 complex | IDA | SGD | 2013-08-07 |
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
|---|
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| CLF1 | SNT309 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| ISY1 | PRP19 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| ISY1 | CLF1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| NTC20 | ISY1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| NTC20 | PRP19 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| NTC20 | PRP19 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| NTC20 | SNT309 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| NTC20 | SYF1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| NTC20 | SYF1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| NTC20 | SYF2 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |