Polarized delivery and incorporation of proteins and lipids to specific domains of the plasma membrane is fundamental to a wide range of biological processes such as neuronal synaptogenesis and epithelial cell polarization. The exocyst complex is specifically localized to sites of active exocytosis and plays essential roles in secretory vesicle targeting and docking at the plasma membrane. Sec3p, a component of the exocyst, is thought to be a spatial landmark for polarized exocytosis. In a search for proteins that regulate the localization of the exocyst in the budding yeast Saccharomyces cerevisiae, we found that certain cdc42 mutants affect the polarized localization of the exocyst proteins. In addition, we found that these mutant cells have a randomized protein secretion pattern on the cell surface. Biochemical experiments indicated that Sec3p directly interacts with Cdc42 in its GTP-bound form. Genetic studies demonstrated synthetically lethal interactions between cdc42 and several exocyst mutants. These results have revealed a role for Cdc42 in exocytosis. We propose that Cdc42 coordinates the vesicle docking machinery and the actin cytoskeleton for polarized secretion.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| SEC5 | located in | cellular bud neck | IDA | SGD | 2013-08-07 | |
| SEC5 | located in | incipient cellular bud site | IDA | SGD | 2013-08-07 | |
| SEC5 | located in | cellular bud tip | IDA | SGD | 2013-08-07 | |
| SEC3 | located in | cellular bud neck | IDA | SGD | 2013-08-07 | |
| SEC3 | located in | incipient cellular bud site | IDA | SGD | 2013-08-07 | |
| SEC3 | enables | small GTPase binding | has input CDC42 | IPI with CDC42 | SGD | 2020-10-25 |
| SEC3 | located in | cellular bud tip | IDA | SGD | 2013-08-07 | |
| CDC42 | involved in | positive regulation of exocytosis | IMP | SGD | 2013-08-07 | |
| CDC42 | involved in | regulation of exocyst localization | IMP | SGD | 2013-08-07 |
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| CDC24 | protein/peptide distribution: abnormal Reporter: Sec3p-GFP | classical genetics | conditional Allele: cdc24-4 | Other | Temperature: elevated temperature, 37 °C Details: Sec3p becomes randomly distributed rather than being polarized at the bud tip | |
| CDC42 | protein/peptide distribution: abnormal Reporter: Sec3p-GFP | classical genetics | conditional Allele: cdc42-201 C6S, D31V, D65G, F90C | Other | Temperature: elevated temperature, 37 °C Details: Sec3p and Sec5p are no longer localized to emerging bud sites, bud tips, and bud necks but either become dispersed throughout the cell or in multiple punctate spots | |
| CDC42 | protein/peptide distribution: abnormal Reporter: Sec3p-GFP | classical genetics | conditional Allele: cdc42-13 R120G | Other | Temperature: elevated temperature, 37 °C Details: Sec3p and Sec5p are no longer localized to emerging bud sites, bud tips, and bud necks but either become dispersed throughout the cell or in multiple punctate spots |
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
|---|
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.