Reference: Naiki T, et al. (2001) Chl12 (Ctf18) forms a novel replication factor C-related complex and functions redundantly with Rad24 in the DNA replication checkpoint pathway. Mol Cell Biol 21(17):5838-45

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Abstract


RAD24 has been identified as a gene essential for the DNA damage checkpoint in budding yeast. Rad24 is structurally related to subunits of the replication factor C (RFC) complex, and forms an RFC-related complex with Rfc2, Rfc3, Rfc4, and Rfc5. The rad24Delta mutation enhances the defect of rfc5-1 in the DNA replication block checkpoint, implicating RAD24 in this checkpoint. CHL12 (also called CTF18) encodes a protein that is structurally related to the Rad24 and RFC proteins. We show here that although neither chl12Delta nor rad24Delta single mutants are defective, chl12Delta rad24Delta double mutants become defective in the replication block checkpoint. We also show that Chl12 interacts physically with Rfc2, Rfc3, Rfc4, and Rfc5 and forms an RFC-related complex which is distinct from the RFC and RAD24 complexes. Our results suggest that Chl12 forms a novel RFC-related complex and functions redundantly with Rad24 in the DNA replication block checkpoint.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Naiki T, Kondo T, Nakada D, Matsumoto K, Sugimoto K
Primary Lit For
RFC5 | RFC2 | RAD24 | RFC3 | CTF18 | RFC4 | DNA replication factor C complex, RFC1 variant | DNA replication factor C complex, CTF18 variant

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 8 entries for 5 genes

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InteractorInteractorAssayAnnotationActionModification
CTF18RFC3Affinity Capture-Westernmanually curatedBait-HitNo Modification
CTF18RFC3Affinity Capture-Westernmanually curatedHit-BaitNo Modification
CTF18RFC4Affinity Capture-Westernmanually curatedBait-HitNo Modification
CTF18RFC4Affinity Capture-Westernmanually curatedHit-BaitNo Modification
RFC2CTF18Affinity Capture-Westernmanually curatedHit-BaitNo Modification
RFC2CTF18Affinity Capture-Westernmanually curatedBait-HitNo Modification
RFC5CTF18Affinity Capture-Westernmanually curatedHit-BaitNo Modification
RFC5CTF18Affinity Capture-Westernmanually curatedBait-HitNo Modification
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