The dye tetraiodofluorescein (TIF) was found to be an effective inhibitor of yeast hexokinase. It is a competitive inhibitor relative to MgATP2- and a noncompetitive inhibitor of glucose binding, a kinetic pattern consistent with the previously proposed random kinetic mechanism. TIF interacts directly with the native dimeric protein to give a difference spectrum with a maximum at 543 nm. Monomeric protein (produced by addition of 0.6 M NaCl) interacts with TIF to give a slightly altered difference spectrum, with the gammamax at 545 nm. The difference spectrum of the dimeric form is not perturbed by the addition of substrates but the absorbance with the monomer is lowered by MgATP2-. The Kd for MgATP2- was estimated to be 7 nM for monomeric hexokinase. These results suggest that results of previous binding studies with hexokinase at high concentrations which have been interpreted as being at variance with kinetic studies are due likely to different conformations of the protein under different experimental conditions.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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