During the conversion of yeast cells to protoplasts, about half of the yeast chitinase was liberated into the medium, an indication that this portion of the enzyme is located in the periplasmic space. At least part of the remaining chitinase appears to be enclosed in vacuoles or in vesicle that co-purify with them, as indicated by a 14-fold enrichment of the enzymatic activity in a vacuole fraction isolated from a protoplast lysate. When protoplasts were incubated in growth medium, part of the chitinase was liberated in growth medium, part of the chitinase was liberated into the medium. It is concluded that yeast chitinase is a secretory enzyme, like invertase and acid phosphatase. The enzyme appears to be stored in vesicles as a prelude to its secretion into the periplasmic space. The possible function of yeast chitinase in cell division is discussed.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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